The iron-fisted Akhandanand Tripathi is a millionaire carpet exporter and the mafia don of Mirzapur. His son, Munna, is an unworthy, power-hungry heir who will stop at nothing to inherit his father's legacy. An incident at a wedding procession forces him to cross paths with Ramakant Pandit, an upstanding lawyer, and his sons, Guddu and Bablu. It snowballs into a game of ambition, power and greed that threatens the fabric of this lawless city.
While Ramakant is tried for the murder of SSP Maurya, Guddu and Golu's claim to the throne is questioned because Kaleen Bhaiya's body is missing. Madhuri vows to fulfill her father's mission of a crime free state.
Guddu and Golu kickstart the business again, but are still looking for a supply of opium which would allow them to establish control over Purvanchal's underworld. In the hunt for the same control, Sharad tries to crack a deal with one of Guddu's old allies, while Madhuri tries to eliminate Guddu.
Guddu cements his power over Mirzapur while Sharad finally makes a very strong ally. Golu receives a threat from Bharat. Ramakant turns down Robin's offer and faces danger in prison. Meanwhile Madhuri strategises to bring Guddu down in other ways.
Ramakant's case is in danger with the appearance of a new witness. In an attempt to save her father's opium business from collapsing, Shabnam turns to Guddu for help. Meanwhile Golu gets some much awaited information about the still missing Kaleen Bhaiya.
Golu plans an attack outside Mirzapur without Guddu. Guddu returns from Nepal to find Golu missing. Sharad senses something is amiss in Siwan and decides to shift his most guarded secret to Jaunpur.
Purvanchal is set ablaze with a gang war between Sharad and Guddu. Madhuri makes strong decisions to break the police-gangster nexus, while a ghost from the past resurfaces. Ramakant finds a purpose in prison, which catches Lala's attention.
Purvanchal is thrown into turmoil following the shocking incident in the jail. Dadda confronts his son, while Sharad and Bharat have a fallout as well. A baithak is called to resolve the gang feud.
Guddu goes down a dark downward spiral as he loses people close to him. A new judge is assigned before Ramakant's final hearing. Meanwhile, Saloni discovers her husband's secrets. IG Dubey is reinstated and is tasked with one mission: Capturing Guddu Pandit.
Beena is compelled to find safety after the fall of Guddu. Madhuri urges Sharad to let go of the past for a better future. Will Shatrughan be able to convince Saloni to keep his secret? A broken Dimpy wants to take matters into her own hands.
Golu returns to Mirzapur only to discover the chaos that ensued in her absence. Madhuri inaugurates Aam Bagh despite the threat of JP Yadav looming large. As Dussehra closes in, there is only one question: Who will ascend the throne of Mirzapur?
